Vol3 Issue1


Vol3 Issue1 _2

posted Jan 17, 2019, 5:35 AM by Yaseen Raouf Mohammed   [ updated Feb 3, 2019, 12:28 AM ]

 COMPARATIVE STUDIES ON EFFECTS OF TIMES AND METHODS OF EXTRACTION ON NITROGEN AND PROTEIN % IN DIFFERENT PLANT SEEDS

 Snowber M. Ahmed


 General Science Department College of Basic Education, Salahaddin University -Erbil, kurdistan Region, Iraq

 ABSTRACT.
A laboratory experiment was carried out in College of Science Education University of Salahaddin –Erbil/Iraq to study the effect of times and methods of extraction on nitrogen and protein percentage in different monocot and dicot seeds. The investigation involved two extraction methods, first was the 5:1sulphoric acid and (potassium sulphate +copper sulphate mixture),while the second method was the 1:1 sulphuric acid and hydrogen peroxide extraction method at three durations of time 30mins, 60mins and 90mins, for each method under programmed temperature. However Lowry methods were used for determination of protein. The results indicated that nitrogen content in monocot grains ranged from 1.22 to 1.68% and from 1.32 to 1.96 % in monocot plant for both methods of extraction respectively , while in dicot plant seeds ranged from 1.84 % and 2.43 to 3.15 % and 3.16% respectively. According to the value of coefficient variation the sulphuric acid and hydrogen peroxide method appear to be more accurate in dicot plants, but in monocot plant the sulphuric acid and CuSO4+K2SO4 mixture method was more homogenous in nitrogen determination. . The correlation coefficient show a significant relationship between
the sulphuric acid and hydrogen peroxide method and Lowry method for determination of protein in plants seeds with correlation coefficient determination r2 =0.936**


 Keywords:
Extraction , Nitrogen, Protein, plants and Kjeldahl method.
  
 References
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[2] Smart, M. M;, R. G. Rada and G. N. Donnermeyer 1983 . Determination of total nitrogen in sediments and plant using persulphate extraction. Evaluation and comparison with Kjeldabl procedure. Water Research 17: 1207-1211.
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Vol3 Issue1 _1

posted Oct 23, 2018, 1:42 AM by Yaseen Raouf Mohammed   [ updated Nov 26, 2018, 5:54 AM ]

 SYNERGISTIC EFFECT OF SILVER NANOPARTICLES COMBINED WITH DIFFERENT ANTIBIOTICS AGAINST MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII STRAIN H72721

 Dana Khdr Sabir


 Department of Medical Laboratory Science, Charmo University, 46023 Chamchamal, Sulaimani, Kurdistan Region, Iraq

 
Abstract. 
Acinetobacter baummannii is aerobic, Gram negative and multidrug resistant bacterium (MDR) which is the main cause of the hospital acquired infection.Using nanoparticle as antimicrobial agents is a promising method to overcome the threat of MDR bacteria, this is not only because the natural effective properties of these particles against the bacterial cells, but it is also unlikely that microorganisms gain resistant against nanoparticles. In this study, silver nanoparticles (Ag-NPs) and synergistic effect of Ag-NPs with different antibiotics were tested against A. baummannii strain H72721 in Tryptic Soy Broth (TSB). Minimum inhibitory concentration (MIC) of each of the ampicillin, kanamycin, gentamycin and clindamycin on the bacterium was 11 mg/ml, 2 mg/ml, 0.5 mg/ ml, and 0.3 mg/ml respectively. In addition, MIC of the Ag-NPs alone towards the bacterial strain was found as (0.75 mg ml- 1). Interestingly, the inhibitory property of each of the tested antibiotics were greatly improved when they were used in combination with Ag-NPs compare to when they were used alone, and in case of each of the ampicillin and clindamycin the bacterial growth reduced 3 folds. Results from this study are strongly suggesting that Ag-NPs could be provide a successful approach to overcome the problem of MDR bacteria and could use as a superficial treatment for A. baummannii’s infections.


 Keywords: 
multidrug resistance, minimuminhibitory concentration, silver nanoparticles


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