Vol3 Issue1

Vol3 Issue1 _2

posted Jan 17, 2019, 5:35 AM by Yaseen Raouf Mohammed   [ updated Mar 25, 2019, 12:44 AM ]


 Snowber M. Ahmed

 General Science Department College of Basic Education, Salahaddin University -Erbil, kurdistan Region, Iraq

A laboratory experiment was carried out in College of Science Education University of Salahaddin –Erbil/Iraq to study the effect of times and methods of extraction on nitrogen and protein percentage in different monocot and dicot seeds. The investigation involved two extraction methods, first was the 5:1sulphoric acid and (potassium sulphate +copper sulphate mixture),while the second method was the 1:1 sulphuric acid and hydrogen peroxide extraction method at three durations of time 30mins, 60mins and 90mins, for each method under programmed temperature. However Lowry methods were used for determination of protein. The results indicated that nitrogen content in monocot grains ranged from 1.22 to 1.68% and from 1.32 to 1.96 % in monocot plant for both methods of extraction respectively , while in dicot plant seeds ranged from 1.84 % and 2.43 to 3.15 % and 3.16% respectively. According to the value of coefficient variation the sulphuric acid and hydrogen peroxide method appear to be more accurate in dicot plants, but in monocot plant the sulphuric acid and CuSO4+K2SO4 mixture method was more homogenous in nitrogen determination. . The correlation coefficient show a significant relationship between
the sulphuric acid and hydrogen peroxide method and Lowry method for determination of protein in plants seeds with correlation coefficient determination r2 =0.936**

Extraction , Nitrogen, Protein, plants and Kjeldahl method.
[1] Shakir, I. M. A. and Adil-K, Kadhir (1989). Determination of total nitrogen from Kjeldahl acid extract using selenium or titanium ( IV ) oxide ( anatase ) with copper II sulphate in tobacco leaves by molecular emission cavity analysis MECA. J. univ. Kuwait sci 16. 261-266.15
[2] Smart, M. M;, R. G. Rada and G. N. Donnermeyer 1983 . Determination of total nitrogen in sediments and plant using persulphate extraction. Evaluation and comparison with Kjeldabl procedure. Water Research 17: 1207-1211.
[3] Dubetz. S. ( 1977 ). Effect of high rates of nitrogen on neepawa wheat grown under irrigation 1- yield and protein content. Can. J. Plant Sci 571 331-336.
[4] Dawood, R. A and Bakheit .B. R. ( 1988 ). Effect of N application at various growth stages on quantity and quality of barley. Assiut Jor. of agri. Sci 19 (5) 181-192.
[5] Baker, R. H and Kadhem M. A. ( 1989 ). Effect of nitrogen on dry matter, chemical composition, and yield of barley and Oats. Iraq. Jor of Agri. Sci 18 (2) : 89-92
[6] Al-zako,J.M.H.(1990) .Effect of VAM on growth of corn and soya bean .M. Sc. Thesis, College of Science University of Salahaddin-Erbil. Iraq .(in arabic).
[7] Darwesh, D. A. (1999). Effect nitrogen source and supplementary irrigation on ammonia volatilization yield and protein content of wheat in different textured soil. Zanco.J.of Salahaddin.Univ .11(2)1999.21-29.
[8] Mustafa,K.K (2003).Effect of different level of nitrogen fertilizer on effectiveness of bacteria Rhizobium leguminosarum strain in Ficia faba plant. Zanco.J.of Salahaddin.Univ.15(2):7-12 .(in arabic).
[9] Sahid ,S.Abd (2010) C0mbination effect of mycorrhizal inoculation and phosphorus fertilizer on yield components and nutrients balance of two wheat species by using modified .M.Sc thesis college of education, Salahaddin Erbil university
[10] Qadir,S.A..2017 Evaluation of the physiological properties and genetic diversity of bread wheat Triticum aestivum L. under drought condition A dissertation college of education. Salahaddin university .
[11] Shakir, I.M.A ; Hussein, F. H. and Jamal, M. A. ( 1988 ). Determination of total nitrogen in fertilizer by molecular emission cavity analysis MECA Iraq. Jor. of Agri Sci (6) 57-67.
[12] Pruden, G., powlsen, D. S. and, D. S. Jenkinson.(1985). Measurement of nitrogen in soil and plant materials. Fertilizer research 6: 205-18
[13] Allen, S. E. Grim H. M and parkson J. A.( 1974 ). Chemical analysis of ecological materials .Blackwell scientific publication London PP:186-187.
[14] Stump, J. M ;, C. B. Christianson and, R. J. Bursh (1985). Aluminum block extraction procedure for determination of total nitrogen in soil containing nitrogen Soil Sci and plant analysis PP:16 1-164 .
[15] Lowry.O.H; N.J.Rosebrough; A.L.Farr and R.J.Randall.(1951),Proteinmeasurement with folin phenol reagent.Journal of .Biol.Chem.193:265-275
[16] Vos,j.(1997).The nitrogen response of potato (Solanum tuberosum L.) in the field: nitrogen uptake and yield, harvest index and nitrogen concentration. Potato Research 40:237-248.
[17]Anglov,T; I. M. Petersen and J. Kristiansen(1999). Uncertainty of nitrogen determination by the Kjeldahl method. Accred Qual Assur . 4: 504–510.
[18]Gajewska .E; M. Wielanek ; K. Bergier and M. Skłodowska.(2009).Nickel-induced depression of nitrogen assimilation in wheat roots. Acta .Physiol. Plant . 31:1291–1300.
[19]Steele,R.G and J,H.Torrie.(1969).Principle and procedure of statistic .McGraw Hill Book Co.Inc.New York.pp:1-530
[20] Barker, A.V and D. J.Pilbeam (2007) handbook of plant nutrition .Taylor and Francis Group Bocan Rotan .London .PP:28-29.

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Vol3 Issue1 _1

posted Oct 23, 2018, 1:42 AM by Yaseen Raouf Mohammed   [ updated Mar 25, 2019, 12:40 AM ]

 Dana Khdr Sabir

 Department of Medical Laboratory Science, Charmo University, 46023 Chamchamal, Sulaimani, Kurdistan Region, Iraq

Acinetobacter baummannii is aerobic, Gram negative and multidrug resistant bacterium (MDR) which is the main cause of the hospital acquired infection.Using nanoparticle as antimicrobial agents is a promising method to overcome the threat of MDR bacteria, this is not only because the natural effective properties of these particles against the bacterial cells, but it is also unlikely that microorganisms gain resistant against nanoparticles. In this study, silver nanoparticles (Ag-NPs) and synergistic effect of Ag-NPs with different antibiotics were tested against A. baummannii strain H72721 in Tryptic Soy Broth (TSB). Minimum inhibitory concentration (MIC) of each of the ampicillin, kanamycin, gentamycin and clindamycin on the bacterium was 11 mg/ml, 2 mg/ml, 0.5 mg/ ml, and 0.3 mg/ml respectively. In addition, MIC of the Ag-NPs alone towards the bacterial strain was found as (0.75 mg ml- 1). Interestingly, the inhibitory property of each of the tested antibiotics were greatly improved when they were used in combination with Ag-NPs compare to when they were used alone, and in case of each of the ampicillin and clindamycin the bacterial growth reduced 3 folds. Results from this study are strongly suggesting that Ag-NPs could be provide a successful approach to overcome the problem of MDR bacteria and could use as a superficial treatment for A. baummannii’s infections.

multidrug resistance, minimuminhibitory concentration, silver nanoparticles

[1] Antibiotic resistance threats in the United States. 2013, Centers for Disease Control and Prevention.
[2] Acinetobacter baumannii infections among patients at military medical facilities treating injured U.S. service members, 2002-2004. MMWR Morb Mortal Wkly Rep, 2004. 53(45): p. 1063-6.
[3] Davis, K.A., et al., Multidrug-Resistant Acinetobacter Extremity Infections in Soldiers. Emerging Infectious Diseases, 2005. 11(8): p. 1218-1224.
[4] Howard, A., et al., Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence, 2012. 3(3): p. 243-50.
[5] Turton, J.F., et al., Comparison of Acinetobacter baumannii Isolates from the United Kingdom and the United States That Were Associated with Repatriated Casualties of the Iraq Conflict. Journal of Clinical Microbiology, 2006. 44(7): p. 2630-2634.
[6] Rice, L.B., Challenges in identifying new antimicrobial agents effective for treating infections with Acinetobacter baumannii and Pseudomonas aeruginosa. Clin Infect Dis, 2006. 43 Suppl 2: p. S100-5.
[7] Alsan, M. and M. Klompas, Acinetobacter baumannii: An Emerging and Important Pathogen. Journal of clinical outcomes management : JCOM, 2010. 17(8): p. 363-369.
[8] Urban, C., S. Segal-Maurer, and J.J. Rahal, Considerations in Control and Treatment of Nosocomial Infections Due to Multidrug-Resistant Acinetobacter baumannii. Clinical Infectious Diseases, 2003. 36(10): p. 1268-1274.
[9] Harding, C.M., S.W. Hennon, and M.F. Feldman, Uncovering the mechanisms of Acinetobacter baumannii virulence. Nature Reviews Microbiology, 2018. 16(2): p. 91.
[10] WHO, Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Geneva: World Health Organization, 2017.
[11] Pal, S., Y.K. Tak, and J.M. Song, Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli. Applied and Environmental Microbiology, 2007.
73(6): p. 1712-1720.
[12] Stoimenov, P.K., et al., Metal Oxide Nanoparticles as Bactericidal Agents. Langmuir, 2002. 18(17): p. 6679-6686.
[13] Li, P., et al., Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology, 2005. 16(9): p. 1912- 1917.
[14] Chudobova, D., et al., Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus. International Journal of Environmental Research and Public Health, 2014. 11(3): p. 3233-3255.
[15] Kohanski, M.A., D.J. Dwyer, and J.J. Collins, How antibiotics kill bacteria: from targets to networks. Nature reviews. Microbiology, 2010. 8(6): p. 423-435.
[16] Abdel Rahim, K.A. and A.M. Ali Mohamed, Bactericidal and Antibiotic Synergistic Effect of Nanosilver Against Methicillin-Resistant Staphylococcus aureus. Jundishapur J Microbiol, 2015. 8(11): p. e25867.
[17] Kim, S.-H., et al., Antibacterial activity of silver-
nanoparticles against Staphylococcus aureus
and Escherichia coli. Korean J. Microbiol. Biotechnol,
2011. 39(1): p. 77-85. http://dx.doi.org/10.31530/17028
[18] Gao, M., et al., Controlled synthesis of Ag nanoparticles with different morphologies and their antibacterial properties. Materials Science and Engineering: C, 2013. 33(1): p. 397-404.
[19] McShan, D., et al., Synergistic antibacterial effect of silver nanoparticles combined with ineffective antibiotics on drug resistant Salmonella typhimurium DT104. Journal of Environmental Science and Health, Part C, 2015. 33(3): p. 369-384.

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