posted Jan 21, 2020, 12:51 PM by Yaseen Raouf Mohammed
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updated Jan 29, 2020, 9:43 AM
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BIOCONTROLLING OF METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS BY USING LACTIC ACID BACTERIA
Tazhan Mohamed Ameen Hama-Kareem
Faculty of Agricultural Science – Sulaimani University, Food science and Human Nutrition department
Zainab Mohamed Nasif Al zubaidy
Faculty of Agricultural Science – Sulaimani University, Food science and Human Nutrition department
Abstract.
The present study was carried out to determine The antibacterial activity of Cell Free Extract of Lactobacillus Spp. Applied
against methicillin resistance Staphylococcus aureus isolated from different type of foods, . Including cake with dressing, dairy products, ready to eat foods, red meat and poultry. A total of 78 samples in local markets in Sulaimani city from March-July/ 2012 tested for susceptibility to methicillin and Biocontrolling of those Samples. Detection of MRSA in this study was conducted to the Staph. Aureus isolates, the results showed that 81 % and 72.4% of the Staph. Aureus were methicillin resistance by disk diffusion methods and by molecular methods (PCR) respectively. The results of Biocontrolling of Staphylococcus aureus isolates by using Lactic Acid Bacteria (Lactobacillus Spp.)showed that Cell free extract (CFE) metabolites of Lactobacillus Spp., that grown in MRS media have inhibitory effect against isolates of MRSA,
indicating the inhibitory effect of Lactobacillus Spp. strains to the growth of these bacteria By using of well diffusion method,
the inhibitory effect of Lactobacillus Spp. Cell free extract was varied according to Staphylococcus aureus isolates the strains and the inhibition zone was ranged from 11 - 18 mm against isolated bacteria.
Methicillin-resistance Lactobacillus Spp, Biochemical tests, DNA Extraction.
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posted Jul 16, 2019, 2:58 AM by Araz Ibrahim Mstafa
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updated Jul 21, 2019, 5:09 AM by Yaseen Raouf Mohammed
]
ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF PROTEINS EXTRACTED FROM MORINDA CITRIFOLIA FRUIT
AbdulMushin M. Shami
Department of Biotechnology, Institute of Genetics Engineering and Biotechnology, University of Baghdad, Baghdad, Iraq
Morinda citrifolia fruit is important medicinal plants which have been used in traditional medicine. Well diffusion assay, minimum inhibitory concentration and the minimum bactericidal concentration (MBC) were used to evaluate antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, methicillin resistance Staphylococcus aureus MRSA and Helicobacter pylori. Free radical scavenging activities DPPH and superoxide dismutase activity assays (SOD) were used to evaluate antioxidant activity. The purpose of the study was to determine antibacterial and antioxidant activities of protein extracted of the Morinda citrifolia fruit. Protein extracts from Morinda citrifolia fruit found to have antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, methicillin resistance MRSA with high antioxidant activities. It could be concluded that the protein extracted of this plant had a good antibacterial and antioxidant effects.
Keywords
Morinda citrifolia; Proteins; Antibacterial properties; Antioxidant
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posted Jun 10, 2019, 5:23 AM by Yaseen Raouf Mohammed
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updated Jun 10, 2019, 5:52 AM
]
INVESTIGATION OF VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS IN ERBIL AND SULAIMANIA CITIES
Srwa Ali Mohammed
Department of Medical Microbiology, Faculty of Science and Health/ Koya University/ Koya KOY45 , Kurdistan Region – Iraq
Rebin A. Omar
Department of Biology, Faculty of Science and Health, Koya University Koya KOY45, Kurdistan Region – Iraq
Rabar S. Muhammed
Assistant Biology at Rizgary Hospital’s Laboratory Erbil/Iraq
Tavga A. Mahmood
Assistant Biology at Sulaimaniyah Teaching Hospital’s Laboratory/ Sulaimanyah/Iraq
ABSTRACT
Twenty three isolates of Staphylococcus aureus were isolated from 100 different samples of (urine, nose, cerebrospinal fluid (CSF), blood, and wound) between January/ 2017 and May/ 2017. Fifty samples for each of Sulaimania and Erbil were identified according to the culture characteristic, morphological and biochemical examinations.The antibiotic susceptibility tests for all isolates were conducted to two antimicrobials agents including [penicillin (p) and vancomycin (VA)] and using the disk diffusion method. The results showed 20 (86.9%) of the S. aureus strains were resistant to penicillin and 3 (13.04%) were vancomycin resistant among MRSA. Two of these isolates that were resistant to vancomycin were obtained from Sulaimania and one of them was obtained from Erbil. The confirmation by PCR was done and a 290bp amplicon product was obtained for VRSA isolates. Only 2 out of 3 of the isolates were corresponding to VRSA, one for each city.
MRSA 1, PCR amplification 2, Staphylococcus aureus 3, vanA gene 4, VRSA 5
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posted Jan 17, 2019, 5:35 AM by Yaseen Raouf Mohammed
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updated Mar 25, 2019, 12:44 AM
]
COMPARATIVE STUDIES ON EFFECTS OF TIMES AND METHODS OF EXTRACTION ON NITROGEN AND PROTEIN % IN DIFFERENT PLANT SEEDS
Snowber M. Ahmed
General Science Department College of Basic Education, Salahaddin University -Erbil, kurdistan Region, Iraq
ABSTRACT.
A laboratory experiment was carried out in College of Science Education University of Salahaddin –Erbil/Iraq to study the effect of times and methods of extraction on nitrogen and protein percentage in different monocot and dicot seeds. The investigation involved two extraction methods, first was the 5:1sulphoric acid and (potassium sulphate +copper sulphate mixture),while the second method was the 1:1 sulphuric acid and hydrogen peroxide extraction method at three durations of time 30mins, 60mins and 90mins, for each method under programmed temperature. However Lowry methods were used for determination of protein. The results indicated that nitrogen content in monocot grains ranged from 1.22 to 1.68% and from 1.32 to 1.96 % in monocot plant for both methods of extraction respectively , while in dicot plant seeds ranged from 1.84 % and 2.43 to 3.15 % and 3.16% respectively. According to the value of coefficient variation the sulphuric acid and hydrogen peroxide method appear to be more accurate in dicot plants, but in monocot plant the sulphuric acid and CuSO4+K2SO4 mixture method was more homogenous in nitrogen determination. . The correlation coefficient show a significant relationship between
the sulphuric acid and hydrogen peroxide method and Lowry method for determination of protein in plants seeds with correlation coefficient determination r2 =0.936**
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posted Oct 23, 2018, 1:42 AM by Yaseen Raouf Mohammed
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updated Mar 25, 2019, 12:40 AM
]
SYNERGISTIC EFFECT OF SILVER NANOPARTICLES COMBINED WITH DIFFERENT ANTIBIOTICS AGAINST MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII STRAIN H72721
Dana Khdr Sabir
Department of Medical Laboratory Science, Charmo University, 46023 Chamchamal, Sulaimani, Kurdistan Region, Iraq
Acinetobacter baummannii is aerobic, Gram negative and multidrug resistant bacterium (MDR) which is the main cause of the hospital acquired infection.Using nanoparticle as antimicrobial agents is a promising method to overcome the threat of MDR bacteria, this is not only because the natural effective properties of these particles against the bacterial cells, but it is also unlikely that microorganisms gain resistant against nanoparticles. In this study, silver nanoparticles (Ag-NPs) and synergistic effect of Ag-NPs with different antibiotics were tested against A. baummannii strain H72721 in Tryptic Soy Broth (TSB). Minimum inhibitory concentration (MIC) of each of the ampicillin, kanamycin, gentamycin and clindamycin on the bacterium was 11 mg/ml, 2 mg/ml, 0.5 mg/ ml, and 0.3 mg/ml respectively. In addition, MIC of the Ag-NPs alone towards the bacterial strain was found as (0.75 mg ml- 1). Interestingly, the inhibitory property of each of the tested antibiotics were greatly improved when they were used in combination with Ag-NPs compare to when they were used alone, and in case of each of the ampicillin and clindamycin the bacterial growth reduced 3 folds. Results from this study are strongly suggesting that Ag-NPs could be provide a successful approach to overcome the problem of MDR bacteria and could use as a superficial treatment for A. baummannii’s infections.
multidrug resistance, minimuminhibitory concentration, silver nanoparticles
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