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posted Jun 10, 2019, 5:23 AM by Yaseen Raouf Mohammed   [ updated Jun 10, 2019, 5:52 AM ]
 INVESTIGATION OF VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS IN ERBIL AND SULAIMANIA CITIES

 Srwa Ali Mohammed

 Department of Medical Microbiology, Faculty of Science and Health/ Koya University/ Koya KOY45 , Kurdistan Region – Iraq

 Rebin A. Omar

 Department of Biology, Faculty of Science and Health, Koya University Koya KOY45, Kurdistan Region – Iraq

 Rabar S. Muhammed

 Assistant Biology at Rizgary Hospital’s Laboratory Erbil/Iraq

 Tavga A. Mahmood

 Assistant Biology at Sulaimaniyah Teaching Hospital’s Laboratory/ Sulaimanyah/Iraq


  ABSTRACT
Twenty three isolates of Staphylococcus aureus were isolated from 100 different samples of (urine, nose, cerebrospinal fluid (CSF), blood, and wound) between January/ 2017 and May/ 2017. Fifty samples for each of Sulaimania and Erbil were identified according to the culture characteristic, morphological and biochemical examinations.The antibiotic susceptibility tests for all isolates were conducted to two antimicrobials agents including [penicillin (p) and vancomycin (VA)] and using the disk diffusion method. The results showed 20 (86.9%) of the S. aureus strains were resistant to penicillin and 3 (13.04%) were vancomycin resistant among MRSA. Two of these isolates that were resistant to vancomycin were obtained from Sulaimania and one of them was obtained from Erbil. The confirmation by PCR was done and a 290bp amplicon product was obtained for VRSA isolates. Only 2 out of 3 of the isolates were corresponding to VRSA, one for each city.


 Keywords:
 MRSA 1, PCR amplification 2, Staphylococcus aureus 3, vanA gene 4, VRSA 5



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