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Vol2 Paper 1

posted Aug 13, 2018, 3:40 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 12:51 AM ]

 Adnan F. AL-Azzawie

 Department of Biology, College of Science, Tikrit University 

Prolactin is a hormone, which is synthesized and secreted from the anterior pituitary gland and various extrapituitary sites under control of a superdistal promoter that contains a single nucleotide polymorphism -1149 G/T. The aim was to assess the association between the extrapituitary -1149 G/T promoter polymorphism of prolactin gene among some Iraqi infertile women and prolactin levels in Salah Al-din province. Blood samples were collected from (82) infertile women and age-matched (40) healthy females. The hormonal tests (FSH, LH & Prolactin) were measured for both groups using Enzyme linked immunosorbent assay (ELISA) Genomic DNA was extracted from blood samples and the presence of single nucleotide polymorphism -1149 G/T of prolactin gene was detected using PCR-RFLP technique. The results showed that the serum hormones levels of infertile women were significantly increased compared with the healthy females. The frequency of (-1149 G) allele and genotype GG were higher in infertile women compared with controls and there was a correlation between elevation of prolactin levels and presence of (-1149 G) allele in the genotypes of infertile women. From the result, it can be concluded that there is a possible association between hormonal levels and infertility. We found a possible relationship between infertility and prolactin -1149 G/T polymorphism. 

Prolactin–1149 G/T Promoter Polymorphism, infertile women, Crude Oil Residue

[1] K. L. Beaker, “Principle and practice of endocrinology and Metabolism , 3rd edition” Lippincott Williams and Wikins, Philadelphia , 918_1015, 2001. 
[2] K. Tamilnadu, and S. Mazher, “Follicle Stimulating Hormone, Luteinizing Hormone and Prolactin Levels in Infertile Women in North Chennai” J. Biosc i. Res., Vol. 1[4]:279-284, 2010. 
[3] Goldman. L, Ausiello. D, “Women health . Cecil medicine, 8th ed” Saunders co.pp: 1026- 1160, 2008. 
[4] R. Blackwell, and R. Azziz, “Essential Reproductive Medicine . 9th ed” MC Graw Hill copanies . pp: 359 – 413, 2006. 
[5] Yasian, L. K, “A study of a number of hematological, biochemical parameters and some hormones in an infertile women in salahaddin governorate” Master thesis, College of Science – Tikrit University, 2010. 
[6] Ajibola, M. Oloruntoba, A. C, Valeria, O. N. A. “Study on Prolactin Hormone and Female Infertility in National Hospital Abuja, Nigeria” IOSR Journal of Dental and Medical Sciences [JDMS] ISSN: 2279-0853,. Volume 2, Issue 2, PP 38-41, [Nov.- Dec. 2012]. 
[7] Murray, R. K. Granner, D. K. Mayes, P. A. and Rodwell, V. W. “Harper’s Biochemistry Twenty” – third edition . alange medical book. USA. pp : 544, 2000.
[8] Muhieddin, K. Yusuf, W. H, Hussein, S, “The endocrine system and reproduction in mammals and birds” Dar Al-Hikma Press Printing Press. Mosul – Iraq, 1990.
[9] Mohammed, M. H. K. “Endocrinology”. Third Edition. University Book House - Al Ain - United Arab Emirates, 2005. 
[10] Bullock , J. Boyle J. and Wang , M. B. “Physiology” 4th ed. Lippincott Williams and Wilkins comp. USA. pp : 588-589, 2001. 
[11] Shenenberger D. hyperprolactine medicine , October 18. e medicine.com. Inc, 2005. 14 
[12] Vera-Lastra, O.et al. “Autoimmunity Reviews” 1, 360–364, 2002. 
[13] Nore, B.F. “Sequence Verifications and Promoter Analysis of The Prolactin Gene”Journal of Zankoy Sulaimani- Part A [JZS-A], 15 [1]. 2013.
[14] Fojtíková, M. et al. “Polymorphism of the extrapituitary prolactin promoter and systemic sclerosis” Rheumatol Int [2010] 30:1691–1693
[15] A, S R M. “Patient fact sheet, Hyperprolactinemia” higway , Birmingham Al abama 35216- 2809 . WWW.asrm .org. .2007. 
[16] Braide, A. Adegoke, O. BAMIGBOWU, E. “Cyclical Changes in Prolactin Levels among Infertile Women Attending University of Port Harcourt Teaching Hospital” J. Appl. Sci. Environ. Manage. Sept , Vol. 15 [3] 425 – 430, 2011.
[17] Menken, J. Trussell, J.and Larsen, U. “Age and infertility” Science.233:1389-1394, 1986.
[18] Snyder , P.J Jacobs, L. S. Utiger, R.D. and William , I. “Thyroid hormone inhibition of the prolactin response to releasing hormone” . J. Clin . Inverst . 52[9] : 2324 – 2329, [2005] 
[19] AL-Azzawie, A.F. “A rapid and non-enzymatic method for genomic DNA extraction from whole blood and some other mammalian tissues”. Journal Tikrit Univ. For Agri. Sci. Vol. [12] No. [4], [2012].
[20] Donn, R.P. Farhan, A., Stevans, A., Ramanan, A. “Neuroendocrine gene polymorphisms and susceptibility to juvenile idiopathic arthritis”. Rheumatology 41, 930–936, 2002. 
[21] Snustad، D. and Simmon, M. J. “Genetics”. 6th ed. John Wiley & Sons، Inc. 2012. 
[22] Micheal, T. MC and Dermott, M.D. [2002]. “Hypothyrodisim. Endocrine Secrets” Third Edition. Hanley & Belfus, INC. 33: 208-212. 
[23] Mclver, B; Romanski, S.A. and Nippoldt, T.B. “Evalution and management of amenorrhea”. Mayo Clinic Proceedings 72[12], 1161- 1169, 1997. 
[24]Bachelot, A. and Binart, N. “Reproductive role of prolactin. Reproduction”.133: 361-369, 2007.
[25] Amiri, D. “Infertility and ovulation screening” Master Thesis, University of Damascus – Syria, 2000. 
[26] Cavallaro, R. Cocchi, F. Angelone, S.M. Lattuada, E. Smeraldi, E. “Cabergoline treatment of risperidone- induced hyperprolactinemia : apllot study” J .Clin. Psy Chiatry.65, 2004.
[27] Ibrahim, A. E. “ Studying of biochemical marks in sera of patients with hyperprolactinemia ” Athesis submitted to college of sciences , Al-Nahrain university .for M.Sc. Degree in Biochemical, 2007. 
[28] Knegtering, H.;Vander, A.E. Castelein, S. Kluiter, H. Vanden Bosch, R.J. “What are the effects of antipsychotic on sexual dysfunctions and medication – induced hypeprolactinemia endocrine Functioning psychoneuro” Endocrindogy 28:109-123, 2003. 15
[29] Jannissle, O. E. “High prevalence of autoimmune thyroiditis in patients with polycystic ovary syndrome” .Eur J. Endocrinol, 150:363-369, 2004. 
[30] Panzan, M. Q. Junior, J. M. Damotta, E. L.;Haapalainen, E . F. Dejesus ;Simoes, M.; Baptista , H .A.; Haidar, M.A. and Baracat, E. C. “Metoclopramide- induced hyperprolactinemia caused marked decline in pinopodes and pregnancy rates in mice” Hum. Reprod . 21: 2514- 2520, 2006. 
[31] Nakajima, M. Terao, T. Iwata, N. and Nakamura, J. “Switching female schizophrenic patients to quetiapine from conventional antipsychotic drugs effects on hyperprolactinemia” pharmacopsychiatry.38:17- 19, 2005. [32] Yilmaz, M.B. Pazarbasi, A. Guzel, A.I. Kocaturk-Sel , S. Kasap, H. Kasap, M. Urunsak, I.F.; Basaran, S. Alptekin, D. and Demirhan, O. [2011]. “Association of serum sex steroid levels and bone mineral density with CYP17 and CYP19 gene polymorphism in postmeno- pausal women in Turkey” Gene. Mol. Res. 10[3]: 1999-2008. 
[33] Guzman-Guzman, I.P., Parra-Rojas, I., Oregón-Romero, E., Ledezma-Lozano, I.Y., Palafox-Sánchez, C.A. “The PADI4 haplotypes are associated with anti-CCP levels in rheumatoid arthritis from Western Mexico”. ClinChem Lab Med 49, S205 Special supp, 2011. 
[34] Kochi, Y., Suzuki, A., Yamada, R., Yamamoto, K. “Ethnogenetic heterogeneity of rheumatoid arthritis-implications for pathogenesis” Nat. Rev. Rheumatol6, 290–295, 2010. 
[35] Stevens, A., Newton, P., Anderson, N.R., Gama, R. “Characterization of a prolactin gene polymorphism and its associations with systemic lupus erythematosus”. Arthritis Rheum. 44 [10], 2358–2366, 2001. 
[36] Montgomery, D.W., Steven, R., Leuis, A. “Prolactin production by immune cells” Lupus 10: 665-675, 2001.

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