Vol2 Paper 1

posted Aug 13, 2018, 3:40 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 12:51 AM ]

 Adnan F. AL-Azzawie

 Department of Biology, College of Science, Tikrit University 

Prolactin is a hormone, which is synthesized and secreted from the anterior pituitary gland and various extrapituitary sites under control of a superdistal promoter that contains a single nucleotide polymorphism -1149 G/T. The aim was to assess the association between the extrapituitary -1149 G/T promoter polymorphism of prolactin gene among some Iraqi infertile women and prolactin levels in Salah Al-din province. Blood samples were collected from (82) infertile women and age-matched (40) healthy females. The hormonal tests (FSH, LH & Prolactin) were measured for both groups using Enzyme linked immunosorbent assay (ELISA) Genomic DNA was extracted from blood samples and the presence of single nucleotide polymorphism -1149 G/T of prolactin gene was detected using PCR-RFLP technique. The results showed that the serum hormones levels of infertile women were significantly increased compared with the healthy females. The frequency of (-1149 G) allele and genotype GG were higher in infertile women compared with controls and there was a correlation between elevation of prolactin levels and presence of (-1149 G) allele in the genotypes of infertile women. From the result, it can be concluded that there is a possible association between hormonal levels and infertility. We found a possible relationship between infertility and prolactin -1149 G/T polymorphism. 

Prolactin–1149 G/T Promoter Polymorphism, infertile women, Crude Oil Residue

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