Vol2 Paper 14

posted Aug 18, 2018, 11:50 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 1:58 AM ]

 Wahran M.Saod

 Department of Chemistry, Faculty of Science, University of Anbar, Ramadi, IRAQ

 Tahseen .A. Zaidan

 Department of Chemistry, Faculty of Science, University of Anbar, Ramadi, IRAQ

 Abdul Wahab .A. Alfaluji

 Fallujah teaching hospital, Fallujah, IRAQ

The aims of this study are: to compare the level of Magnesium in sera of patients infected with HBV and healthy participants, and to evaluate the efficiency of analytical techniques (Inductively Coupled plasma- Mass spectrometry (ICP-MS), Atomic Absorption Spectroscopy that are currently used to detect Magnesium in Patients’ human sera. One hundred sixty patients’ samples and thirty healthy individuals were investigated in this study. All the patients included in this research were tested positive for HBS Ag test and their specimens were examined by profile test which include (HBS Ab, HBe Ag, HBe Ab, HBc Ab ,(IgM-IgG), HCV and HIV) and also viral load (HBV PCR) with unit (copies/ml) were carried to test the patients sera. Magnesium levels was determined by using Atomic Absorption Spectroscopy inductively oupled plasma- Mass spectrometry (ICP-MS), the measured readings of AAS and ICP-MS for Magnesium was compared. The findings of this study show that the concentration range of Magnesium element between (1.59±0.30 mg\l) to (1.74±0.17mg\l).
Serum Magnesium has shown decrease in mean serum concentration in patients with chronic hepatitis B compared to healthy individuals. Moreover, (AAS and ICP-MS) analytical techniques have same efficiency in measuring the Magnesium concentration.

Hepatitis, B, Serum, Magnesium and ICP-MS.

[1] FX. Bosch, J. Ribes, R. Cleries and M. Diaz “Epidemiology of Hepatocellular Carcinoma” Clin Liver Dis, vol. 9, 2005, pp; 191–211.
[2] SM. Alavian “ Immunization: an important strategy to control hepatitis B. Hepat Mon “ vol. 6 [1], 2006, pp.; 3 – 5.
[3] EE. Mast, CM. Weinbaum, AE. Fiore, MJ. Alter, BP. Bel and L. Finelli “ A Comprehensive Immunization Strategy to Eliminate Transmission of Hepatitis B Virus Infection in The United States—Mrecommendations of the Advisory Committee on Immunization Practices [ACIP] Part II: Immunization of Adults “ MMWR , vol. 55 , [RR-16] , 2006 , pp ; 1 – 33
[4] GM. McQuillan, PJ. Coleman, D. Kruszon-Moran, LA. Moyer and SB. Lambert “ Margolis HS. Prevalence of Hepatitis B Virus Infection in the United States” The National Health and Nutrition.
[5] Examination Surveys “1976 through 1994. [See comments], Am J Public Health, vol. 89, 1999, pp.; 14 – 18.
[6] L. Jules “Dienstag Drug Therapy Hepatitis B Virus Infection” N Engl J Med, vol. 359, 2008 ¸pp; 1486 – 500.
[7] Das and MK. Maini “Innate and Adaptive Iimmune Responses in Hepatitis B Virus Infection” Dig Dis Sci, A Vol. 28 [1] 2010, p: 32 – 126.
[8] RP. Beasley “Hepatitis B Virus. The Major Etiology of Hepatocellular Carcinoma “Cancer, vol. 61¸1988, pp; 1942 – 1956.
[9] SB. Rosalki, N. Mcintyre¸ “Biochemical investigations in the management of liver disease”. Oxford textbook of clinical hepatology, 2nd ed. New York; Oxford University press; 503- 521[1999].
[10] M. Abdul Aziz , R. D. Bikha , A. S. Syed Zulfiquar , R. Qasim , A. M. Sikander , B. Marya , A. Q. Ghulam and S. Waqas” Metabolic Investigations in Patients With Hepatitis B and C” 169 World J Gastroenterol 2010 February 7 , vol. 16[5] , pp; 603-607, ISSN 1007-9327 , [2010].
[11] C. E. Housecroft, A. G. Sharpe “ Inorganic Chemistry [3rd ed.]” Prentice Hall, pp; 305–306, [2008].
[12] J. Vormann “ Magnesium: Nutrition and Metabolism. Molecular Aspect of Medicine” Vol. 23, pp; 27, [2003].
[13] C. Dean “The Magnesium Mericle” Ballantine Books, New York, [2007].
[14] S. Baig, AA. Siddiqui, W. Ahmed, H. Qureshi and A. Arif “The Association of Complex Liver Disorders With HBV Genotypes Prevalent in Pakistan” Virol J, vol. 4, 2007¸ pp: 128.
[15] F. Bianchi, M.Maffini, A. Mangia, E.Marengo and C.Mucchio” Experimental design optimization for the ICP-AES determination of Li, Na, K, Al, Fe, Mn and Zn in human serum Journal of Pharmaceutical and biomedical Analysis 43, 2007: pp659-665.
[16] PD. Mayne “ Biochemical Investigation of Renal, Water and Electrolyte Disorders. In: Clinical Chemistry in Diagnosis and Treatment “ Mayne PD [ed]. 6th edition. ELBS, [1996].
[17] VS. Venkataseshan, K. Lieberman, DU. Kim, SN. Thing and S. Dikmai “Hepatitis B-Associated Glomerulonephritis” Medicine [Baltimore], vol. 69, 1990, pp: 200- 216.
[18] P. Angeli, F. Wong, H. Watson and P. Gines “ Hyponatremia in Cirrhosis: Results of a Patient Population Survey “ Hepatology, vol. 44, 2006¸ pp: 1535-1542.
[19] M. Koivisto, P. Valta, K. Hockerstedt and L. Lindgren “Magnesium Depletion Inchronic Terminal Liver Cirrhosis” Clin Transplant, vol. 16 [5], 2002¸pp: 325-328.
[20] B. Das, P. Chandra and K.V. Thimmaraju “Serum Magnesium Level in Patients with LiverCirrhosis” Int J Biol Med Res, vol. 2[3], 2011¸pp: 709-711.

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