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Vol2 Paper 5

posted Aug 13, 2018, 5:21 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 1:04 AM ]
CHARACTERIZATION OF SOME BACTERIA AND MEASUREMENT OF IL12 CONCENTERATION IN PATIENTS WITH GALL BLADDER INFLAMMATION.


 Sama Jwad Kadhim AL-zuwainni

 Department of Biology College of Science for Women University of Babylon

 ABSTRACT
 The most prevalent complication of gallstone is chronic cholecystitis. The aim of the present study was to determine the responsible microorganisms association in gall bladder inflammation in patients undertook cholecystectomy and determine the relationship of serum interlukin-12 concentration and some hematological parameter with gall bladder infection. Cholecystectomy was achieved in 107 patients. Collected samples transferred to laboratory in appropriate media, then cultured on selective media to isolate the possible causative bacteria. The levels of interleukin-12, PCV, WBC, HB. Bacterial growth was shown at 94.4% of all cultured samples , of which 87.6% gram negative bacteria (E. coli, Pseudomonas, Enterobacter, Klebsiella, salmonella, shigella, Acintobacter, Citrobacter, Proteus).and 12.4% gram positive bacteria (Enterococcus feacales, Staphylococcus.areuse, Staphylococcus epidermis, Bacillus, Streptococcus pneumonia, and Streptococcus viridians .The results explain increase in levels of interleukin-12 and variation in hematological parameters compared with control. The study also revealed three types of stones depending upon their color (36 cholesterol, 26 mixed, 34 pigment). High percentage of bacteria was isolated from pigment stone, whereas cholesterol stone shows no bacterial growth.


 Keywords:
Cholecystectomy, E.coli, interleukin-12, stones, bacterial.



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