Vol2 Paper 5

posted Aug 13, 2018, 5:21 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 1:04 AM ]

 Sama Jwad Kadhim AL-zuwainni

 Department of Biology College of Science for Women University of Babylon

 The most prevalent complication of gallstone is chronic cholecystitis. The aim of the present study was to determine the responsible microorganisms association in gall bladder inflammation in patients undertook cholecystectomy and determine the relationship of serum interlukin-12 concentration and some hematological parameter with gall bladder infection. Cholecystectomy was achieved in 107 patients. Collected samples transferred to laboratory in appropriate media, then cultured on selective media to isolate the possible causative bacteria. The levels of interleukin-12, PCV, WBC, HB. Bacterial growth was shown at 94.4% of all cultured samples , of which 87.6% gram negative bacteria (E. coli, Pseudomonas, Enterobacter, Klebsiella, salmonella, shigella, Acintobacter, Citrobacter, Proteus).and 12.4% gram positive bacteria (Enterococcus feacales, Staphylococcus.areuse, Staphylococcus epidermis, Bacillus, Streptococcus pneumonia, and Streptococcus viridians .The results explain increase in levels of interleukin-12 and variation in hematological parameters compared with control. The study also revealed three types of stones depending upon their color (36 cholesterol, 26 mixed, 34 pigment). High percentage of bacteria was isolated from pigment stone, whereas cholesterol stone shows no bacterial growth.

Cholecystectomy, E.coli, interleukin-12, stones, bacterial.

[1] Greenberger N.J., Isselbacher K.J. [1998]. Diseases of the gallbladder and bile ducts. In: Fauci S.A., Braunwald E., Isselbacher K.J., et al.[eds].Harrison’s Principles of Internal Medicine.
McGraw-Hill, New York.
[2] Csendes A.; Burdiles P.; Maluenda F.; Diaz C.; Csendes P. and Mitru N. [1996a]. Simultaneous bacteriologic assessment of bile from gallbladder and common bile duct in control subjects and patients with gallstones and common duct stones. Arch Surg 131: 389-394.
[3] Vitetta L, Best SP, Sali A. [2000].Single and multiple cholesterol gallstones and the influence of bacteria. Med Hypotheses.55 [6]:502-6.
[4] Chandran P., Kuchhal N. K., Garg P. and Pundir C. S. [2007].an extended analysis of gall stone. Ind .J. Clin. Biochem. 22[2]:145-150.
[5] Eslami G., Nowruzi J., Fllah F., Goudarzi H., Hakemivala M., and Jahangiri S. [2007]. Detection of bacteria responsible for gallbladder inflammation and gallstones. Iran .J.Clin.Inf. Dis.2 [3]:139-141.
[6] Al-khafeji F. A. [2006]. Bacteriological and Clinical study of gallbladder stones and bills after 58 cholecystectomy .Msc. Thesis. Collage of medicine, Babyl university
[7] Swidsinski, A.; and Lee S.P. [2001].the role bacteria in gall stone pathogenesis. Frontiers in Bioscience. 6 e93-103.
[8] MacFaddin, J.F. [2000]. Biochemical tests for identification of medical bacteria.3rd ed. Awolters Kluwer Company. Baltimore,: 78-424.
[9] Finegold, S M.and Baron E J. [1986].Diagnostic microbiology; 7th. Ed.; the C.V. Mosby Company U.S.A.:175-201.
[10] Baggiolini, M.; Dewald, B. and Moser, B. [1994]. Interleukin-8 and related chemotactic cytokines-CXC and CC chemokines. Adv. Immunol. , 55: 97- 179.
[11] Lewis, S.M.; Bain, B.J. and Bates, I. [2001] Dacie and Lewis practical haematology. 9th ed., Livingston. Churchill.
[12] Niazi, A.D. [2004]. Statistical Analysis in Medical Research. 2nd Ed Coll.of Med., Nahrain Univ. Baghdad. P: 73-98.
[13] Vaishnavi C., Singh S., Kochhar R. [2005]. Prevalence of Salmonella enteric serovar Typhiin bile and stool of patients with biliarydiseases and those requiring biliary drainage for other purposes. Jpn J Infect Dis.58:363-5.
[14] Horn G. [1965]. Observations on the aetiology of cholelithiasis. BriMed J. 2: 732.
[15] Moore EW. [1984].The role of calcium in the pathogenesis ofgallstones. Hepatology; 4: 228- 43.
[16] Jayanthi V., Palenivela U. C., Prasanthi R., Mathew S., and Srinivason V.1998. Composition of gallstones in Coimbatore District of Tamil Nadu Sate. Ind.J. Gastroenterol.17:134-5.
[17] Bedirli A, Sakrak O, Sözüer EM, et al. [2001]. Factors effecting the complications in the natural history of acutecholecystitis Hepatogastroenterology. 48[41]:1275-8.
[18] Pundir CS, Chaudhary R, Rani K, Chandran P, Kumari M,Garg P.[2001].Chemical analysis of biliary calculi in Haryana. Ind J.Surg. 63: 370-73.
[19] Ti JK, Yuen R. [1985].Chemical composition of biliary calculi inrelation to pattern of biliary disease in Singapore. Br J Surg. 72: 556-8.
[20] Csendes A., Mitru N., Maluenda F., et al. [1996b]. Counts of bacteria and pyocites of choledochal bile in controls andin patients with gallstones or common bile duct stones with or without
acut cholangitis. Hepatogastroenterology.43 [10]:800-6.
[21] Csendes A., Becerra M., Burdiles P., Demian I., Bancalari K.and Csendes P. [1994]. Bacteriological studies of bile from gallbladder in patients with carcinoma of the gallbladder, cholelithiasis, common bile duct stones and no gallstones disease. Eur J Surg160: 363-367.
[22] Chijiiwa K., H. Ichimiya, S. Kuroki, A. Koga and F. Nakayama.[1993]. Late development of cholangiocarcinoma after the treatment of hepatolithiasis. SurgGynecolObstet177: 279-282.
[23] Bonnet F, Neau D, Viallard JF, et al. [2001]. Clinical andlaboratory findings of cytomegalovirus infection in 115hospitalized no immunocompromised adults. Ann MedInterne [Paris].
[24] Tseng LJ, Tsai CC, Mo LR, et al. [2000]. Palliativepercutaneous transhepatic gallbladder drainage ofgallbladder empyema before laparosc cholecystectomy. Hepatogastroenterology.47
[25] Miranda RT, Gimeno AE, Rodriguez TF, et al. [2001]. Acutecholecystitiscaused by Brucellamelitensis: case report and review. J Infect.42 [1]:77-8.
[26] Lau SM, Peng MY, Chang FY. [2000]. Outcomes ofAeromonas bacteremia in patients with different types of underlying disease. J MicrobiolImmunol Infect.33 [4]:241-7.
[27] Brook I. [1989]. Aerobic and anaerobic microbiology of biliary tract disease.J ClinMicrobiol 27:2373-2375.
[28] Roitt, IBrostoff, J and Male, D. [2001]. Immunology 6thed.W.B Saunders.
[29] Temblay, J.N.Bertelli, E. Arques, J.L., Regoli, M. and Nicoletti, C. [2007]. Production of IL 12 by peyer patch dendritic cells is critical for the resistance to food allergy J.Allergy clin. Immunol. 120[3]:659-665.
[30] Wu, H.P.; Chang, C.F. ans Lin, C.Y. [2003]. Predicitive inflammatory parameters in the diagnosis of acute appendicitis in children. Acta Paediatr. Taiwan. , 44:227–731.

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