Vol2 Paper 6

posted Aug 13, 2018, 5:41 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 1:05 AM ]
INHIBITION OF BIOFILM PRODUCTION IN MULTI-DRUG RESISTANT KLEBSIELLA PNEUMONIA BY EXTRACT OF SOME PLANTS IN THE FLORA OF KOYA CITY.


 Srwa Ali Mohammed

 Faculty of Science and Health, Koya University, Danielle Mitterrand Boulevard, Koya KOY45, Kurdistan Region-Iraq

 Shwan Kamal Rachid
 
 College of Education and Natural Sciences Charmo University Chamchamal, 46023, Kurdistan-Iraq 

 Komar Research Center (KRC) Komar University of Science and Technology Qularaisi- Sarchinar, Sulaimani, 46001, Kurdistan-Iraq

ABSTRACT 
 This paper investigates the effects of sub-Inhibitory Concentrations (SICs) of some plants in the flora of Koya city/Kurdistan region of Iraq on biofilm production in Klebsiella pneumonia. 0.1-10 mg of the plant organic extracts used against growth and biofilm production in microtiter plates measured spectroscopically. While the SIC effect of each of Eugenia caryophyllata (Clove) and Quercus infectoria oliv has significantly reduced biofilm formation, no negative effect on the bacterial growth was investigated. Interestingly, while the synergistic effect of some plant extract mixes (like Poamgranet mixed with tetracyclin and Poamgranet with ampicillin. Salix candida mixed with refampicin and Salix candida with neomycin) at SICs level reduced biofilm production, mixtures from the other plants extract (like leafs of Salix candida and Punica granatum) have significantly increased biofilm expression in a multidrug resistant Klebsiella pneumonia. Similar synergistic effects were recorded when the bacteria grown in presence of the plants extract SICs mixed with sub-lethal concentration of some antibiotics in comparison to the effects of solely individual plant extracts.


 Keywords: 
Biofilm, Klebsiella pneumonia, plant extracts, antimicrobial agents.



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