Vol2 Paper 7

posted Aug 13, 2018, 6:01 AM by Yaseen Raouf Mohammed   [ updated Sep 4, 2018, 1:08 AM ]
 Β-CATENIN AND PROSTACYCLIN RECEPTOR DIFFERENTIALLY REGULATE HAIR FOLLICLE DERMAL PAPILLA CELLS.


 Karzan Ghafur Khidhir

 Department of Biology, College of Science, University of Sulaimani, Sulaimani, Iraq.

ABSTRACT
Human hair follicle dermal papilla (DP) cells are widely used to study the molecular mechanisms which underlie hair development. Prostamides, prostaglandins and their analogues are potent stimulators of hair growth, but, knowledge on their mechanism of action is limited. This study aims to improve understanding of the mechanism of latanoprost action in the human DP cells and determines the role of β-Catenin signalling and prostacyclin receptor (IP) in the pathway. DP cells were isolated after 10 days of culture with/without latanoprost and pooled for RNA extraction. PCR was carried out to confirm expression of β-Catenin and IP genes; and expression levels were compared using quantitative real-time PCR analysis. PCR analysis identified β-Catenin and IP genes in scalp DP cells and Quantitative real-time PCR demonstrated elevated expression of β-Catenin and IP in DP cells treated with latanoprost, and the most significant increase was achieved in cells treated with 100 nM Latanoprost. Identification of β-Catenin and IP genes in the DP cells and up regulation of these genes in cells treated with latanoprost indicate that latanoprost acts through Wnt/β-Catenin signalling pathway and prostacyclin receptor. So the β-Catenin and prostacyclin receptors play important role in signalling pathways within human hair follicle DP cells.


 Keywords:
 β-Catenin, Dermal Papilla, Hair, Quantitative Real-Time PCR, Prostacyclin.



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